| 0 | Meyrick / Legge / Rojas | Claro/TracFone DeltaWing Racing (USA) | | DeltaWing | DWC13 | | Elan | P |
| 007 | Nielsen / Wittmer / Davis | TRG-AMR (USA) | Kevin Buckler | Aston Martin | V12 Vantage | GT3 | Aston Martin | GTD |
| 01 | Hand / Pruett / Dixon | Chip Ganassi Racing with Felix Sabates | | Riley | Mk XXVI | | Ford | P |
| 07 | Long / Miller | Mazda Motorsports (USA) | Sylvain Tremblay | Mazda | Prototype | | Mazda | P |
| 3 | Garcia / Magnussen / Briscoe | Corvette Racing (USA) | | Chevrolet | Corvette C7 | .R | Chevrolet | GTLM |
| 4 | Gavin / Milner / Briscoe | Corvette Racing (USA) | | Chevrolet | Corvette C7 | .R | Chevrolet | GTLM |
| 5 | Fittipaldi / Barbosa / Bourdais | Action Express Racing, Coyote DP | | Chevrolet | Corvette DP | | Chevrolet | P |
| 8 | Schultis / van der Zande / Hedlund / Popow | Starworks Motorsport | | Oreca | FLM 09 | | Chevrolet | PC |
| 10 | Taylor / Taylor / Angelelli | No. 10 Konica Minolta Corvette DP for Wayne Taylor Racing | | Chevrolet | Corvette DP | | Chevrolet | P |
| 11 | Cumming / Junqueira / Menezes | RSR Racing | | Oreca | FLM 09 | | Chevrolet | PC |
| 16 | Mowlem / Drumwright / Drissi / Yount | BAR1 Motorsports | | Oreca | FLM 09 | | Chevrolet | PC |
| 17 | Sellers / Henzler / Long | Team Falken Tire | | Porsche | 991 RSR | I | Porsche | GTLM |
| 22 | Keen / MacNeil / Davis | Alex Job Racing | | Porsche | 991 GT America | | Porsche | GTD |
| 23 | James / Riberas / Farnbacher | Team Seattle/Alex Job Racing | | Porsche | 991 GT America | | Porsche | GTD |
| 24 | Luhr / Edwards / Klingmann | BMW Team RLL (USA) | | BMW | Z4 | GTE | BMW | GTLM |
| 25 | Farfus / Auberlen / Werner | BMW Team RLL (USA) | | BMW | Z4 | GTE | BMW | GTLM |
| 31 | Cameron / Curran / Papis | Action Express Racing, Coyote DP | | Chevrolet | Corvette DP | | Chevrolet | P |
| 33 | Keating / Bleekemolen / Bleekemolen | Riley Motorsports (USA) | Bill Riley | Dodge | Viper | SRT GT3-R | Viper | GTD |
| 38 | French / Mee / Daly | Performance Tech Motorsports | | Oreca | FLM 09 | | Chevrolet | PC |
| 44 | Potter / Lally / Renauer | Magnus Racing | | Porsche | 991 GT America | | Porsche | GTD |
| 45 | Cosmo / Thorne / Thompson | Flying Lizard Motorsports | | Audi | R8 LMS | Grand-Am | Audi | GTD |
| 48 | von Moltke / Haase / Miller | Paul Miller Racing | | Audi | R8 LMS | Grand-Am | Audi | GTD |
| 52 | Kimber-Smith / Guasch / Palmer | PR1/Mathiasen Motorsports | | Oreca | FLM 09 | | Chevrolet | PC |
| 54 | Bennett / Braun / Lazzaro | Core Autosport | | Oreca | FLM 09 | | Chevrolet | PC |
| 60 | McMurry / Pew / Negri | Michael Shank Racing with Curb/Agajanian (USA) | | Ligier | JS P2 | | Honda | P |
| 62 | Fisichella / Kaffer / Vilander | Risi Competizione (USA) | | Ferrari | 458 | Italia GT | Ferrari | GTLM |
| 63 | Segal / Sweedler / Bell | Scuderia Corsa (USA) | | Ferrari | 458 | Italia Grand-Am | Ferrari | GTD |
| 64 | Serra / Westphal / Cressoni | Scuderia Corsa (USA) | | Ferrari | 458 | Italia Grand-Am | Ferrari | GTD |
| 70 | Nunez / Bomarito / Tremblay | Mazda Motorsports (USA) | Sylvain Tremblay | Mazda | Prototype | | Mazda | P |
| 73 | Pumpelly / Lindsey / Snow | Park Place Motorsports | | Porsche | 991 GT America | | Porsche | GTD |
| 85 | Miller / Goikhberg / Mitchell | JDC/Miller Motorsports | | Oreca | FLM 09 | | Chevrolet | PC |
| 88 | Popow / Rayhall / Falb | Starworks Motorsport | | Oreca | FLM 09 | | Chevrolet | PC |
| 90 | Westbrook / Valiante / Rockenfeller | VisitFlorida.com Racing | | Chevrolet | Corvette DP | | Chevrolet | P |
| 93 | Lawrence / Carter / Goossens | Riley Motorsports (USA) | Bill Riley | Dodge | Viper | SRT GT3-R | Viper | GTD |
| 97 | Marsal / Palttala / Priaulx / Johnson | Turner Motorsport | | BMW | Z4 | E89 GT3 | BMW | GTD |
| 911 | Tandy / Pilet / Lietz | Porsche North America (USA) | Jon Bennett | Porsche | 991 RSR | I | Porsche | GTLM |
| 912 | Bamber / Bergmeister / Makowiecki | Porsche North America (USA) | Jon Bennett | Porsche | 991 RSR | I | Porsche | GTLM |